Laboratory Equipment & Services Information System by Centralized Laboratory Management Office (CeLMO)

High-performance liquid chromatography (HPLC) is an analytical technique to separate, identify, and quantify components in a mixture. It is the main chromatography technique used in most laboratories worldwide.

In column chromatography, a solvent drips through a column filled with an adsorbent under gravity. HPLC is a highly improved form of column chromatography. A pump forces a solvent through a column under high pressures of up to 400 atmospheres. The column packing material or adsorbent or stationary phase is typically a granular material of solid particles such as silica or polymers.