A flow cytometer is an analytical instrument that measures and characterises cells or particles in a fluid stream using lasers and optical detectors. It provides high-throughput, multiparametric data on cell size, complexity, and fluorescence, making it essential for applications in immunology, cell biology, and clinical diagnostics.
Applications
CytoFLEX Fluorochrome Panel
| Laser | Channel | Detector | Common Fluorochromes |
|---|---|---|---|
| 405 nm (Violet) | V450 | 450/45 | BV421, Pacific Blue |
| 405 nm (Violet) | V525 | 525/40 | BV510 |
| 488 nm (Blue) | B530 | 530/30 | FITC |
| 488 nm (Blue) | B585 | 585/42 | PE |
| 488 nm (Blue) | B690 | 690/50 | PerCP, PI |
| 638 nm (Red) | R660 | 660/20 | APC |
| 638 nm (Red) | R712 | 712/25 | APC-R700 |
| 638 nm (Red) | R780 | 780/60 | APC-Cy7 |
The flow cytometer is used to analyse physical and chemical characteristics of cells or particles as they pass individually through a laser beam. It provides rapid, quantitative measurements of cell size, granularity, and fluorescence, enabling applications such as immunophenotyping, cell counting, and viability assessment.