Laboratory Equipment & Services Information System by Centralized Laboratory Management Office (CeLMO)

Pyrosequencing with the PyroMark platform integrates detection and quantification of genetic variation into one system and outperforms other sequence-based solutions for cost-effective analysis of targeted short DNA sequences. The Pyrosequencing sequencing-by-synthesis technology is suitable for a range of applications where quantifiable sequence data are critical for characterization of complex DNA modifications.

Pyrosequencing sensitively quantifies somatic mutation frequencies and DNA methylation levels at both CpG and non-CpG (CpN) sites and presents these results as a percentage. In addition, insertion-deletions (indels) and unknown sequence variants are characterized and single nucleotide polymorphisms (SNPs) are analyzed to resolve sample heterozygosity. It is possible to assay several contiguous sequence variants in a single run.