Laboratory Equipment & Services Information System by Centralized Laboratory Management Office (CeLMO)

An ELISA Reader (Multi-Wavelength) is a microplate spectrophotometer designed to measure optical density (OD) in enzyme-linked immunosorbent assays (ELISA) and other absorbance-based applications. The multi-wavelength feature allows detection at multiple wavelengths for enhanced accuracy and flexibility in different assays.

An ELISA Reader (Multi-Wavelength) is used for measuring absorbance in enzyme-linked immunosorbent assays (ELISA) and other optical density-based applications. It provides accurate and reproducible quantification of biological and chemical substances.

1. Clinical & Medical Diagnostics

Detects infectious diseases (HIV, dengue, hepatitis, COVID-19).

Measures hormone levels (e.g., insulin, thyroid hormones).

Monitors autoimmune diseases and cancer biomarkers.

2. Pharmaceutical & Biomedical Research

Used in drug discovery to test compound effects.

Measures enzyme activity and protein interactions.

Assesses antibody and antigen binding in immunological studies.

3. Food Safety & Environmental Testing

Detects toxins, allergens, and contaminants in food samples.

Analyzes water and environmental pollutants (e.g., heavy metals, pesticides).

4. Molecular Biology & Biochemistry

Quantifies proteins, DNA, and RNA using assays like Bradford, Lowry, or BCA.

Measures enzyme kinetics and metabolic activities.

How to Use an ELISA Reader:

Prepare Microplate – Load samples into a 96-well or 384-well plate.

Set Parameters – Choose the wavelength(s) based on the assay protocol.

Insert the Plate – Place the microplate into the reader.

Measure Absorbance – The device detects optical density (OD) at selected wavelengths.

Analyze Results – Use software to calculate concentration, standard curves, and cut-off values.

Clean & Maintain – Remove the microplate and clean the instrument for future use.