Laboratory Equipment & Services Information System by Centralized Laboratory Management Office (CeLMO)

Gas Chromatography (GC) with Flame Ionization Detector (FID) is an analytical technique used for the separation and detection of volatile organic compounds (VOCs). The FID is a highly sensitive detector that measures the presence of organic compounds by burning them in a hydrogen-air flame and detecting ionized carbon fragments.

Gas Chromatography (GC) with Flame Ionization Detector (FID) is used for separating and analyzing volatile organic compounds. The FID detects ionized carbon fragments from organic compounds as they are burned in a hydrogen-air flame.

How to Use GC-FID:

Prepare the System:

Ensure the GC is properly installed and calibrated.

Check and fill the carrier gas (e.g., helium, nitrogen), hydrogen, and air supplies.

Perform leak checks to prevent gas loss.

Prepare the Sample:

Dilute the sample if necessary.

Load the sample into a clean, properly prepared vial.

Use a syringe or autosampler to inject the sample into the GC system.

Set the Operating Parameters:

Adjust the oven temperature for optimal separation.

Set the carrier gas flow rate for efficient column performance.

Define the FID settings, including hydrogen, air, and makeup gas flow rates.

Run the Analysis:

Inject the sample and start the GC-FID system.

Monitor the chromatogram for peak separation and identification.

Record and analyze the data.

Shutdown and Maintenance:

Allow the system to cool before turning off the gas supply.

Clean the injection port, column, and FID regularly.

Perform routine maintenance to ensure accuracy and longevity.