Laboratory Equipment & Services Information System by Centralized Laboratory Management Office (CeLMO)

A Class II Biosafety Cabinet (BSC) is a ventilated enclosure designed to provide personnel, product, and environmental protection when handling hazardous biological materials. It is commonly used in microbiology, virology, and pharmaceutical labs.

A Class II Biosafety Cabinet (BSC) is used for handling infectious materials while providing protection to personnel, products, and the environment. Proper usage is crucial to maintaining safety and preventing contamination.

1. Pre-Use Preparation

✔ Check Airflow & Alarms – Ensure the cabinet is functioning properly with no alarm indicators.
✔ Wear Personal Protective Equipment (PPE) – Lab coat, gloves, and safety goggles (if necessary).
✔ Disinfect the Work Surface – Use 70% ethanol or appropriate disinfectant.
✔ Arrange Materials Properly – Place necessary items inside to minimize movement and airflow disturbance.
✔ Allow Cabinet to Stabilize – Let it run for at least 5 minutes before use.

2. Proper Work Practices

✔ Work at the Center of the Cabinet – Keep all activities at least 4 inches from the front grille.
✔ Minimize Disruptions – Avoid rapid arm movements and opening/closing the sash frequently.
✔ Use Correct Hand Techniques – Move hands in and out slowly to maintain airflow stability.
✔ Avoid Blocking Air Grilles – Do not place objects on front or rear grilles.
✔ Work with Minimal Equipment – Only essential tools should be inside the BSC.

3. Post-Use Procedures

✔ Seal & Dispose of Waste Properly – Place biohazard waste in autoclavable bags before removal.
✔ Disinfect Work Area – Wipe down surfaces with an appropriate disinfectant.
✔ Run Cabinet for 5-10 Minutes – Allow airflow to clear contaminants before shutting off.
✔ Remove PPE & Wash Hands – Prevent cross-contamination.

4. Maintenance & Safety Checks

✔ HEPA Filter Monitoring – Regular checks and replacement as needed.
✔ Airflow Certification – Annual testing to ensure safe operation.
✔ UV Light Use (if applicable) – Only when the cabinet is not in use and never as a substitute for proper disinfection.