Laboratory Equipment & Services Information System by Centralized Laboratory Management Office (CeLMO)

The Alpha Cycler thermal cyclers are standalone systems with flexible block options (96 or 384 well formats being available). The range includes both a single block, the AC-1, a dual block variant, the AC-2 and a quad block variant, the AC-4. The Alpha Cyclers range of block options allow for use of the most commonly used consumables including 0.2ml x 96 well plates, 0.2ml tubes, 0.2ml strip tubes and 384 well plates. All combinations of base unit and block include a gradient feature, manually adjustable heated lid and programming features such as touchdown, active sample cooling, advanced reporting and health check options as standard to allow for most applications to be performed easily.

Temperature Block temperature range 4°C (10°C during cycling) to 100°C Block uniformity (at 55°C) ≤±0.3°C Temperature accuracy (at 55°C) ≤±0.25°C Gradient availability Between 10°C and 100°C Maximum gradient 29°C on a 96 well block and 19°C on a 384 well block Minimum gradient 1°C Column uniformity with a 10°C gradient ≤±0.3°C Final store 4°C to 100°C Temperature set point adjustment 0.1°C Heating/Cooling Rate Maximum heating rate 3.4°C/s Minimum heating rate 0.1°C/s Maximum cooling rate 1.0ºC/s Minimum cooling rate 0.1ºC/s Heated lid Selectable heated lid temperature 35 to 115°C or off Lid uniformity ±5°C Pre-heat lid Yes Warm up time from ambient <2 min Over-temperature cut-out Yes Regulated heated lid pressure Manually adjustable The heated lid is only operational if the block temperature is set above 35°C. Above figures are quoted at an ambient temperature of 20°C ±2°C Programming Program interface 10” and 7” HD touch screen Operation system Android Maximum number of programs stored 1000 Maximum number of stages per program 25 Maximum number of steps 10 Maximum number of cycles per stage 99 Programmable ramp rate Yes, 0.1°C/s steps Maximum hold time 4h 59m 59s Minimum hold time 1s Program Wizard Yes Active sample cooling Yes, 4°C Advanced reporting options Yes Touchdown Yes Incremented/decremented temperature Yes Incremented/decremented time Yes Pause facility Yes Program naming Alpha numeric plus symbols Password protection Yes Keyword selection Yes Run completion time Yes Auto resume on power failure Yes, always, never or user-defined Oligonucleotide Tm calculator Yes, based on the Nearest-Neighbour method1 Software updates Free of charge from www.coleparmer.com Communication ports USB port on the front of the AC-1 Connection for USB memory stick USB port on side of the AC-2 / AC-4 Connection for USB memory stick Type B USB port on rear of unit For Internal use only

To amplify segments of DNA via the Polymerase Chain Reaction (PCR). The device has a thermal block with holes where tubes holding the PCR reaction mixtures can be inserted.