Laboratory Equipment & Services Information System by Centralized Laboratory Management Office (CeLMO)

Muse™ System The Muse™ System consists of a compact, portable, and easy-to-use cell analyzer, software, and optimized reagents. The Muse™ Cell Analyzer uses patent-pending, miniaturized fluorescent detection and micro-capillary technology to deliver quantitative cell analysis of both suspension and adherent cells 2 to 60 μm in diameter. The software includes a dedicated module for each assay, as well as a tools for verifying instrument performance and cleaning the instrument’s fluid system. Convenient mix-and-read assays are optimized for accuracy and convenience. The available assays include: • Count & Viability • Annexin V & Dead Cell • Cell Cycle

Sample collection, acquisition and analysis of results is achieved in less than six steps for most assays. The user interface is designed to be intuitive, so you spend less time with experimental setup and analysis for the critical cell health assays you need most. Novel, Miniaturized Flow Technology The Muse® Cell Analyzer uses patent-pending, miniaturized fluorescent detection and microcapillary technology to deliver truly accurate, precise and quantitative cell analysis compared to other methods. Laser-based fluorescence detection of each cell event can evaluate up to 3 cellular parameters – cell size (forward scatter) and 2 colors (detected in the red and/or yellow channels). The system uses a microcapillary and miniaturized optics, which occupy one-tenth the space of a typical cytometer. This means that the instrument occupies only an 8 in x 10 in (20 cm x 25 cm) footprint. A green diode laser is used for excitation, and a uniquely designed series of retro-reflective lenses provide maximum light capture and sensitivity. Highly Intuitive Touchscreen Interface Muse® features a highly intuitive touchscreen interface that allows simple step-by-step operation, so easy that no flow expertise is required to run assays. The touchscreen prompts you through simple on-screen instructions and guides you though sample loading to simple setting adjustments to results—in just a few steps! Software Muse® software is designed to be simple enough for any user to follow setup instructions and achieve sample acquisition, on the first use. Assay-specific software modules include sample graphs to make population gating setup foolproof. Results are displayed in both graphical and statistical formats specific to each application, making analysis unambiguous. Muse Cell Analyzer Reported Statistics on Viable Cells. Data output for the Muse® Count and Viability Assay. Healthy Jurkat cells were mixed with heat-killed Jurkat cells and stained with Muse® Count & Viability Reagent, and then analyzed on the Muse® Cell Analyzer. Data output include summary data (not shown) and optional dot plots (shown here). Reported statistics include viable cells/mL, % viability, and the total cells/mL. The left hand dotplot shows viability vs cell size; the right hand plot shows viability vs nucleated cells.

The Muse® Cell Analyzer Is used to determine viability and total cell count. It uses miniaturized fluorescence detection and microcapillary cytometry to deliver single-cell analysis, which is highly quantitative — especially when compared with traditional bulk methods like Western blot.