Laboratory Equipment & Services Information System by Centralized Laboratory Management Office (CeLMO)

The BioPhotometer plus converts the measured absorbance values into concentration results. Dependent on the method the results can be calculated via fixed factors, standards, or curve calibration. In addition to the results the device also displays the absorbance values and some other important details, e.g. the common absorbance quotients for nucleic acid calculations. Sample dilutions can also be included in the evaluation. Other special evaluation procedures are provided for specific method groups. For example, when calculating the concentration of dyed nucleic acids the frequency of incorporation related to the amount of nucleic acid can also be calculated.

The BioPhotometer plus is designed for use with cuvettes such as the Eppendorf UVette®, but is also optimized for measurement of small volumes through the use of microliter cells such as Hellma® TrayCell. Applications Quantification of nucleic acids (dsDNA, ssDNA, RNA, Oligo) Quantification of proteins (280 nm, Bradford, Lowry, BCA) Cell density measurement (OD600) NEW - Incorporation rate of fluorescent dyes (dye 550/650, e.g., Cy-3, Cy-5) NEW - Enzymatic assays: endpoint detection (e.g., of enzyme activities or cell biological assays) NEW - Direct absorbance measurement of single wavelengths without any calculation (230 nm, 260 nm, 280 nm, 340 nm, 405 nm, 490 nm, 550 nm, 595 nm, 650 nm)

The BioPhotometer plus, Eppendorf offers a compact UV/Vis photometer for use in molecular biology, biochemistry and cell biology. The photometer provides instant, out-of-the-box access to 32 routine methods, of which 9 methods are freely programmable: Measurement of DNA, RNA and protein concentration (UV and colorimetric) Incorporation rate of fluorescent molecules (550 nm/650 nm), e. g., for microarray experiments Enzymatic assays (e.g., peroxidase, alkaline phosphatase, β-galactosidase) Optical density of cells (OD 600)