The Kjeldahl method is a standard method for determining the nitrogen content in food and animal feed as well as in soil and water samples. It can be used flexibly and universally, including with inhomogeneous sample material, and always provides reliable results. The Gerhardt (For Protein analysis) comes with 3 major components, ie Kjeldatherm, Turbosog and Vapodest for digestion and distillation of samples.
In the Kjeldahl procedure, after digestion in concentrated sulfuric acid, the total organic nitrogen is converted to ammonium sulfate. Ammonia is formed and distilled into boric acid solution under alkaline conditions. The borate anions formed are titrated with standardized hydrochloric acid, by which is calculated the content of nitrogen representing the amount of crude protein in the sample.
Nitrogen determination according to the Kjeldahl method is used to calculate the protein content in samples.